FastQCFastQC Report
Thu 6 Apr 2017
G124_G125_M20M21M22.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124_G125_M20M21M22.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58810685
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3392510.5768526586622822Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN888130.15101507489667906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT611000.042.55418411
AGCGTCG609500.042.52674510
CGTCGTG612000.042.49210712
GTCGCCG405800.042.27314844
GATCGGA624600.041.9325751
GAGCGTC622950.041.6256949
TGGTCGC401850.041.60539242
ATCGGAA626950.041.534772
GTCGTGT628350.041.5080613
TCGTGTA625450.041.45204514
GATCTCG472750.041.43200334
CGTGTAG629050.041.36487215
GTGGTCG426800.041.0904241
GGTCGCC419150.040.88444543
AGAGCGT638050.040.7572638
ATCTCGG487650.040.59482235
TCGGAAG645350.040.56453
TCTCGGT477850.040.36572636
TCGGTGG501100.040.33268438
AAGAGCG643000.040.312537
Produced by FastQC (version 0.11.2)