FastQCFastQC Report
Tue 2 Aug 2016
G124-G125-M20M38-NEBNext21_GTTTCG-_AC8KUAANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext21_GTTTCG-_AC8KUAANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20790793
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG635420.3056256680541238Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN318020.1529619384888301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT116000.040.9538211
CGTCGTG116450.040.83061612
AGCGTCG116250.040.7304410
GTCGCCG78250.040.61735544
TGGTCGC77450.039.84519242
CGTGTAG120800.039.5652315
GATCGGA120700.039.5204161
TCGTGTA120700.039.45615814
GTCGTGT121500.039.38281213
GATCTCG91200.039.3072634
GAGCGTC121450.039.0766839
GGTCGCC82500.038.8180343
GTGGTCG83450.038.77269741
ATCGGAA122800.038.7410932
ATCTCGG95650.037.9156835
AGAGCGT126400.037.7369468
TCTCGGT94450.037.60742636
TCGGTGG99550.037.3387738
TCGGAAG128400.037.2923133
AAGAGCG127400.037.2528347
Produced by FastQC (version 0.11.2)