FastQCFastQC Report
Fri 19 May 2017
CEBPb_All_Reps_Combined.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCEBPb_All_Reps_Combined.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92129597
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5155960.5596420876561524Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1108860.12035871599438343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG651450.042.78751444
TGGTCGC643300.041.9758342
GTGGTCG678950.041.5283341
GGTCGCC669800.041.46736543
GATCTCG738450.041.38039834
TCGGTGG777200.040.8261638
TCTCGGT735100.040.70536836
ATCTCGG766500.040.4746235
CGGTGGT725750.040.35363839
CGTGTAG1087800.040.12770515
GATCGGA1109800.040.088611
CTCGGTG750900.039.9881137
ATCGGAA1115950.039.753612
CGTCGTG1095900.039.7180412
GCGTCGT1096900.039.64384511
AGCGTCG1102050.039.59098410
TCGGAAG1133250.039.2693523
TCGTGTA1104650.039.03690714
GTCGTGT1116000.039.03387513
GAGCGTC1116150.038.9357079
Produced by FastQC (version 0.11.2)