FastQCFastQC Report
Fri 19 May 2017
CEBPb_TCPO_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCEBPb_TCPO_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44920305
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1900130.4230002445441989TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT586450.042.3220444
TCTCGTA543250.041.86781343
CGTCTGA640050.041.6000716
CCGTATC329450.041.4944738
ACGTCTG645800.041.26379815
ATCTCGT551650.041.14148342
AGTTCCG378550.040.973334
CGTATCT334900.040.94370339
TCCGTAT342450.040.86972437
GATCGGA648000.040.4177861
TATCTCG343600.040.13191241
ACACGTC673000.039.62205513
ATCGGAA661850.039.612552
GTTCCGT390300.039.41444435
TCGGAAG670100.039.1443253
AATCTCG227550.039.0656441
CGGAAGA671250.038.8610574
TTCCGTA395250.038.83193636
GCACACG699300.038.19517511
CACGTCT702600.037.99369414
Produced by FastQC (version 0.11.2)