FastQCFastQC Report
Fri 19 May 2017
CEBPb_TCPO_Reps_Combined.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCEBPb_TCPO_Reps_Combined.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44920305
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2962960.6596037137325759Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN542500.12076943823066207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG361700.042.9943444
TGGTCGC357750.042.3253442
GTGGTCG378300.041.92808541
GGTCGCC369750.041.843943
GATCTCG410850.041.6715934
TCGGTGG433800.041.2702838
TCTCGGT414150.040.8152136
CGGTGGT403500.040.72281339
ATCTCGG427200.040.6433135
CTCGGTG416350.040.52157237
CGTGTAG604550.040.03764715
GATCGGA617500.040.032461
ATCGGAA621150.039.670812
CGTCGTG616150.039.31728412
TCGGAAG628000.039.3049663
AGCGTCG616950.039.2313110
GCGTCGT616300.039.2226511
GTCGTGT621800.038.92085313
TCGTGTA615850.038.85937514
GAGCGTC624550.038.730079
Produced by FastQC (version 0.11.2)