FastQCFastQC Report
Fri 19 May 2017
CEBPb_Veh_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCEBPb_Veh_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47209292
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1340800.2840118847789541TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC496800.041.95691344
TCGTATG501250.041.6517443
CTCGTAT483500.041.59652342
TCTCGTA448250.041.45836641
CGTCTGA518450.041.2170816
ACGTCTG523650.040.9003615
GATCGGA517350.040.8244321
ATCTCGT458850.040.3991240
ATCGGAA529050.040.0139242
ACACGTC539800.039.72152313
AATCTCG307700.039.63794339
TCGGAAG537800.039.3176733
CGGAAGA543300.038.8022734
GCACACG565050.037.85719711
CACACGT568400.037.81482712
CACGTCT572650.037.5159214
TATCTCG170350.037.32198339
GAACTCC603700.035.28556421
CGACGCT27150.034.28393617
CAGTCAC623200.033.96074727
Produced by FastQC (version 0.11.2)