FastQCFastQC Report
Fri 19 May 2017
CEBPb_Veh_Reps_Combined.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCEBPb_Veh_Reps_Combined.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47209292
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2193000.46452719519708113Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN566360.1199679080126853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG279850.042.57849544
TGGTCGC278200.041.60608742
GTGGTCG294800.041.02472741
GGTCGCC289000.041.00226243
GATCTCG324750.040.9291534
TCGGTGG342550.040.2371638
CGTGTAG475000.040.20762315
GATCGGA483650.040.2063181
CGTCGTG473150.040.17804312
GCGTCGT472600.040.17310311
TCTCGGT325150.040.14268536
AGCGTCG475900.040.05233810
ATCGGAA486250.039.8824042
CGGTGGT318250.039.75057639
ATCTCGG339950.039.72056635
TCGTGTA479000.039.2963614
GAGCGTC482700.039.283629
GTCGTGT485450.039.2510313
TCGGAAG496500.039.2300953
CTCGGTG333900.039.17772337
Produced by FastQC (version 0.11.2)