FastQCFastQC Report
Fri 19 May 2017
RXR_All_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXR_All_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54051413
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT730370.13512505214248516TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1092750.042.9598121
ATCGGAA1097650.042.6556662
CGTATGC990700.042.43901444
CGTCTGA1102700.042.41415816
ACGGCTA979900.042.39666432
CGGCTAC980250.042.30491333
TCGTATG997400.042.16290743
ACGTCTG1106350.042.1369615
ACACGTC1110000.042.0545413
TCGGAAG1117850.041.9183853
TCACGGC997100.041.74752430
CGGAAGA1119900.041.7312134
GTCACGG1002300.041.5705529
CATCTCG939600.041.55738439
AGTCACG1010500.041.5138328
CACGGCT1004550.041.33207731
CACGTCT1129350.041.30819314
CACACGT1147250.040.74915312
GCACACG1148800.040.73713311
CCGTACG96850.040.3130833
Produced by FastQC (version 0.11.2)