FastQCFastQC Report
Fri 19 May 2017
RXR_Veh_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXR_Veh_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23922003
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT730370.30531306262272434TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG94050.041.44317633
CGTACGT94350.041.35604534
ACCGTAC95950.040.6688832
CGTAATC86500.040.12495438
GATCGGA102550.040.1243171
GTACGTA98400.039.67632335
CACCGTA100600.038.92000231
CTCGTAT96550.038.7976244
ATCGGAA106150.038.7032852
TACGTAA100950.038.47776436
TCTCGTA91100.038.19721643
CGTCTGA108900.037.99504516
AATCTCG91850.037.7406841
ACGTAAT95350.037.3927837
TCACCGT105900.037.05393630
GTCACCG106750.037.04743629
ACGTCTG111600.036.9377615
ACACGTC112550.036.567713
ATCTCGT95450.036.4547542
TCGGAAG114500.036.053843
Produced by FastQC (version 0.11.2)