FastQCFastQC Report
Fri 19 May 2017
RXR_Veh_Reps_Combined.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXR_Veh_Reps_Combined.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23922003
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG487800.20391269075587024Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN438070.1831242977437968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG93300.041.11161810
CGTCGTG93050.041.09942612
GCGTCGT94500.040.5445211
GATCGGA97500.039.8274271
GTCGCCG68250.039.26378344
TCGTGTA97750.038.93486414
ATCGGAA99550.038.878182
GAGCGTC98950.038.8089689
TGGTCGC68050.038.60170442
GTCGTGT100200.038.4475613
CGTGTAG100100.038.15011215
GTGGTCG71350.038.08217641
GATCTCG79200.038.0382634
AGAGCGT103950.036.9846738
TCGGAAG105650.036.645673
GGTCGCC73850.036.34583743
ATCTCGG83450.036.28410735
AAGAGCG106050.036.0463077
TCGGTGG86000.035.64193738
CGGAAGA110000.034.9016384
Produced by FastQC (version 0.11.2)