FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M1M20-NEBNext13_AGTCAA-_AC8KUAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext13_AGTCAA-_AC8KUAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66913892
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1790180.26753487900539397Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT785050.041.07579411
CGTCGTG828400.040.62691512
TCGTGTA817300.040.2718414
CGTGTAG835600.039.5754615
GTAGGGA843350.039.4388918
GTCGTGT842200.039.3466613
AGCGTCG784650.039.2779110
TGTAGGG888150.038.49099717
GAGCGTC807750.038.461429
AGAGCGT820300.038.4115688
GATCTCG531500.037.82583634
GATCGGA855300.037.608061
TAGGGAA870400.037.5567119
ATCTCGG539950.037.43992635
GTCGCCG442350.037.39005744
GTGTAGG903950.037.2007116
ATCGGAA869800.036.872622
AAGAGCG832400.036.6709027
TCTCGGT545200.036.4940436
GTAGATC580100.036.3751131
Produced by FastQC (version 0.11.2)