FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M1M20-NEBNext14_AGTTCC-_AC8KUAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext14_AGTTCC-_AC8KUAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14649498
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT1503861.0265607736183178TruSeq Adapter, Index 14 (97% over 44bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT769320.52515110074079No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA412080.2812929152930701No Hit
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT251340.17156901895204874TruSeq Adapter, Index 14 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT374700.041.98195344
TCCGTAT346000.041.95163337
AGTTCCG394400.041.89909734
CCGTATC335850.041.89471438
GTTCCGT389950.041.72328635
CGTATCT337650.041.54273639
TTCCGTA389900.041.40669336
TCTCGTA346200.041.0227243
TATCTCG339850.040.82411641
CGTCTGA411950.038.96693416
ACGTCTG419050.038.7897515
GAACTCC433100.038.0338121
CAGTTCC449450.037.5009833
CACGTCT453750.037.4719614
ACACGTC451450.037.45151513
ACAGTTC449900.037.38282432
GTATCTC378000.037.27661540
CCAGTCA467650.037.1488726
AGTCACA469650.037.0954428
CAGTCAC470000.037.0771727
Produced by FastQC (version 0.11.2)