FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M1M20-NEBNext15_ATGTCA-_AC8KUAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext15_ATGTCA-_AC8KUAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12169082
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT894880.7353718218021704TruSeq Adapter, Index 15 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT456550.3751720959723996No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA234960.19307947797541344No Hit
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT147510.12121703181883399TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT230150.041.23460444
AATCTCG205650.040.666141
TCTCGTA218600.039.1626143
ACGTCTG261250.038.52212515
CGTCTGA255750.038.4472316
ATGTCAG271950.037.68850734
GAATCTC225550.037.64301340
ACATGTC276800.037.2765132
GTCAGAA267750.037.2198736
ACACGTC279250.037.1869513
TCACATG281600.036.94515230
GTCACAT284600.036.9050129
TGTCAGA275700.036.88880535
CACGTCT284500.036.74287414
CACATGT284200.036.59987631
GAACTCC272000.036.29681821
CATGTCA288100.036.09471533
AGAATCT236700.035.8145939
TCTGAAC278300.035.74305318
CTGAACT275650.035.70353719
Produced by FastQC (version 0.11.2)