FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M1M20-NEBNext16_CCGTCC-_AC8KUAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext16_CCGTCC-_AC8KUAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5048191
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG16900.033.17268435
GATCGAC7600.032.1361417
ATCGACT7950.031.55164718
ACCCGTC18600.031.20672632
TCACCCG19550.030.4775330
CTCGTAT17500.029.81950444
TCGACTG7900.028.96613719
CACCCGT20400.028.56096631
GTCCCGA19200.028.28226736
GACTGAT21150.026.94231813
TCCCGAT17900.026.03710637
TGATCGG10800.025.87404416
ACTGATC22500.025.42583314
TCTCGTA17500.025.4157843
CGACTGG7850.025.2252620
CCCGTCC23300.025.00443333
CCGTCCC23500.024.69807834
CCCGATC18200.024.18237338
GATCGAG8500.024.06136123
ATGATCG7800.023.97776215
Produced by FastQC (version 0.11.2)