FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M1M20-NEBNext16_CCGTCC-_AC8KUAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext16_CCGTCC-_AC8KUAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5048191
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT14100.032.1439711
CGTCGTG15300.031.77766812
TCGAAAT15100.030.765435
CGTATGC12300.029.7183841
AATCGAA15800.029.537063
GTCGTGT15950.029.40284513
TCGTGTA16150.029.03353314
CGAAATG16050.027.5671756
CGTGTAG17250.027.56332415
GTAGGGA17500.027.53816618
TAGTATC10400.027.50236110
AGCGTCG16000.026.95231810
GAGCGTC16850.025.4618789
AAATCGA17650.025.4347442
TGTAGGG18950.025.20053117
TATGCCG10950.025.1295343
TTAGTAT12400.024.6631649
TAGGGAA19400.024.4993819
GACACAT19350.024.46402713
AGAGCGT18150.024.3635548
Produced by FastQC (version 0.11.2)