FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M1M20-NEBNext18_GTCCGC-_AC8KUAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext18_GTCCGC-_AC8KUAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6870843
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220821.7768125395966696No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT920491.3397046039328797TruSeq Adapter, Index 18 (97% over 40bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA589780.8583808420597007No Hit
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT157160.22873466909373422TruSeq Adapter, Index 18 (97% over 40bp)
CTCAAAATTCTGTGACAGATTTTTGGTCAAGTTGTTTCCATTAAAAAGTA84710.12328909276489071No Hit
GTCTTTTTGTTATTTTGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84650.12320176723583992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT230000.042.75481444
ACGTCCG249700.042.49752432
GTCACGT254350.042.43935429
CCGCACA248050.042.2532136
CGCACAT218000.042.17662437
AGTCACG258200.042.09034328
GTCCGCA252700.041.65901634
CATCTCG210800.041.51247841
TCTCGTA215100.041.0202743
TCACGTC267700.040.9295930
CAGTCAC271900.040.5114527
GCACATC220400.040.48401638
CGTCCGC264550.040.26670533
ACATCTC222150.040.08590340
CACGTCC269950.039.93681331
TCCGCAC264800.039.9048735
GCGGAAG35550.039.8868373
AGCGGAA32050.039.7820662
GAGCGGA31900.039.4420851
CGTCTGA269200.038.8269116