FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M1M20-NEBNext18_GTCCGC-_AC8KUAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext18_GTCCGC-_AC8KUAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6870843
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1381282.0103501127881978No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT426420.6206225349640503No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG422940.6155576542791037Illumina Single End PCR Primer 1 (100% over 50bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA123630.17993425260917764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA204500.042.73897614
GTCGTGT206100.042.61599313
CGTGTAG208450.042.53032715
GTAGGGA210000.042.09778618
AGAGCGT200400.041.936598
TGTAGGG218750.041.70436517
ATCTCGG120100.041.6827335
GGTCGCC96900.041.58596443
TCTCGGT118300.041.50135436
CGTCGTG218050.041.4774912
GATCGGA207650.041.3646281
GAGTGTA168500.041.25518427
GATCTCG120850.041.23851834
GCGTCGT209500.041.2296411
TCGGTGG129150.041.0140238
ATCGGAA207150.040.942822
CTCGGTG121400.040.87804837
GAGCGTC203400.040.7562039
GTGGTCG104800.040.70426641
GTGTAGG220850.040.66494416
Produced by FastQC (version 0.11.2)