FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M21M42-NEBNext12_CTTGTA-_AC8KUAANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext12_CTTGTA-_AC8KUAANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11231164
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1579911.4067197309201434No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1206811.0745190792334613No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1107970.9865139534958264TruSeq Adapter, Index 12 (100% over 50bp)
TTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399770.35594707725753094No Hit
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC323760.2882693191907802TruSeq Adapter, Index 12 (98% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291770.2597860738210216No Hit
GTGCCAGTTACCTTGTTTTTTTTTGGTCTTTTTGTTATTTTGTCTTTTTT171020.15227272970103545No Hit
GCCAGTTACCTTGTTTTTTTTTGGTCTTTTTGTTATTTTGTCTTTTTTTT168530.15005568434402702No Hit
CTCAAAATTCTGTGACAGATTTTTGGTCAAGTTGTTTCCATTAAAAAGTA130310.11602537368344013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC259300.042.4511244
CTCGTAT249550.042.10911642
TCGTATG261000.042.0819343
ACGTCTG244900.041.78188315
ACACGTC250300.041.70704313
AATCTCG227550.041.53098339
CGTCTGA243200.041.394916
CACACGT253800.040.93556212
TCTCGTA232850.040.87920441
GCACACG255650.040.78635411
GATCGGA178200.040.6440961
TGTAATC230250.040.480636
CGGAAGA265600.040.4627534
CCTTGTA264300.040.44976433
CACGTCT263450.040.11019514
GTAATCT234550.040.10398537
AGTCACC276450.040.0654728
ATCGGAA184050.039.9668162
GTCACCT274350.039.92214629
AGCGGAA55500.039.7021452