Basic Statistics
Measure | Value |
---|---|
Filename | G126-G127-M21M42-NEBNext12_CTTGTA-_AC8KUAANXX_L006_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 11231164 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 157991 | 1.4067197309201434 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 120681 | 1.0745190792334613 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC | 110797 | 0.9865139534958264 | TruSeq Adapter, Index 12 (100% over 50bp) |
TTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 39977 | 0.35594707725753094 | No Hit |
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC | 32376 | 0.2882693191907802 | TruSeq Adapter, Index 12 (98% over 50bp) |
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 29177 | 0.2597860738210216 | No Hit |
GTGCCAGTTACCTTGTTTTTTTTTGGTCTTTTTGTTATTTTGTCTTTTTT | 17102 | 0.15227272970103545 | No Hit |
GCCAGTTACCTTGTTTTTTTTTGGTCTTTTTGTTATTTTGTCTTTTTTTT | 16853 | 0.15005568434402702 | No Hit |
CTCAAAATTCTGTGACAGATTTTTGGTCAAGTTGTTTCCATTAAAAAGTA | 13031 | 0.11602537368344013 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 25930 | 0.0 | 42.45112 | 44 |
CTCGTAT | 24955 | 0.0 | 42.109116 | 42 |
TCGTATG | 26100 | 0.0 | 42.08193 | 43 |
ACGTCTG | 24490 | 0.0 | 41.781883 | 15 |
ACACGTC | 25030 | 0.0 | 41.707043 | 13 |
AATCTCG | 22755 | 0.0 | 41.530983 | 39 |
CGTCTGA | 24320 | 0.0 | 41.3949 | 16 |
CACACGT | 25380 | 0.0 | 40.935562 | 12 |
TCTCGTA | 23285 | 0.0 | 40.879204 | 41 |
GCACACG | 25565 | 0.0 | 40.786354 | 11 |
GATCGGA | 17820 | 0.0 | 40.644096 | 1 |
TGTAATC | 23025 | 0.0 | 40.4806 | 36 |
CGGAAGA | 26560 | 0.0 | 40.462753 | 4 |
CCTTGTA | 26430 | 0.0 | 40.449764 | 33 |
CACGTCT | 26345 | 0.0 | 40.110195 | 14 |
GTAATCT | 23455 | 0.0 | 40.103985 | 37 |
AGTCACC | 27645 | 0.0 | 40.06547 | 28 |
ATCGGAA | 18405 | 0.0 | 39.966816 | 2 |
GTCACCT | 27435 | 0.0 | 39.922146 | 29 |
AGCGGAA | 5550 | 0.0 | 39.702145 | 2 |