FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M21M42-NEBNext1_ATCACG-_AC8KUAANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext1_ATCACG-_AC8KUAANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12890883
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG241660.18746582371432585Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTAAAATCAGCCAGATGGGACTGGAGTTCAGACGTGTGCTCTTCCGAT239220.18557301311322116TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT108750.040.35986311
CGTCGTG112900.039.1281412
CGTGTAG118700.038.72876715
TCGTGTA120450.038.5728514
GTAGGGA114000.038.46418818
AGCGTCG113000.038.2003210
GTCGTGT117050.037.62535513
GATCGGA126950.037.386351
AGAGCGT117250.037.3302048
TGTAGGG121000.037.2733517
GATCTCG72750.037.2398334
GAGCGTC115950.037.1715359
ATCGGAA129350.036.223142
TTCCGAT96250.036.1967944
ATCTCGG78150.036.08694535
TAGGGAA122350.035.67289719
GTAGATC86550.035.66031331
TCAGACG94850.035.46690430
TCTCGGT77000.035.4455436
AAGAGCG120750.035.3197147
Produced by FastQC (version 0.11.2)