FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M21M42-NEBNext2_CGATGT-_AC8KUAANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext2_CGATGT-_AC8KUAANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8270309
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC595750.7203479337954604TruSeq Adapter, Index 2 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210840.25493606103472066No Hit
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC174590.21110456695148877TruSeq Adapter, Index 2 (98% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99570.120394534230825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGA142050.041.9750330
TCGTATG146100.041.367743
CGATGTA140250.041.3527834
CGTATGC146750.041.27438744
CACCGAT143500.041.15239331
CCGATGT141600.041.05196833
GTCACCG149800.040.9628229
CTCGTAT144250.040.76997442
ACGTCTG138700.040.65402215
ACCGATG144900.040.49650232
ACACGTC142300.040.3060813
TATCTCG129250.039.97072639
CGTCTGA139300.039.86248416
TCTCGTA132050.039.38966841
CACGTCT147400.039.3449214
CACACGT146200.039.23027412
CGGAAGA151500.039.206634
GATGTAT133800.038.6943735
GATCGGA99550.038.453111
GCACACG149650.038.3817511
Produced by FastQC (version 0.11.2)