FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M21M42-NEBNext2_CGATGT-_AC8KUAANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext2_CGATGT-_AC8KUAANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8270309
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG273630.3308582545102003Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170460.2061107994876612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT114400.042.17936711
CGTCGTG117850.041.2399312
TCGTGTA123300.040.82288714
AGCGTCG116900.040.80772810
GTAGGGA118200.040.54782518
GTCGTGT118750.040.5177413
CGTGTAG123200.040.4744915
GAGCGTC119500.040.0302939
GATCGGA129250.040.00981
TGTAGGG124150.039.736817
GATCTCG73300.039.5936434
AGAGCGT123400.039.5316938
ATCTCGG77000.039.16141535
ATCGGAA132000.039.062672
GTAGATC83950.038.7476831
TAGGGAA122650.038.49872219
TCTCGGT76200.038.1771936
GTGTAGG128500.038.10301216
GTCGCCG63050.038.0210544
AAGAGCG125050.037.9726377
Produced by FastQC (version 0.11.2)