FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M21M42-NEBNext4_TGACCA-_AC8KUAANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext4_TGACCA-_AC8KUAANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8042616
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC365970.45503850985798655TruSeq Adapter, Index 4 (100% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC106270.13213362418397198TruSeq Adapter, Index 4 (98% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85960.10688064679452558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC92450.040.94289844
TCGTATG92450.040.63362543
GATCGGA61050.039.881231
CTCGTAT91000.039.73425342
AATCTCG81900.039.5023239
ACACGTC89500.039.38782513
ACGTCTG88950.039.3343215
CGTCTGA88250.039.0724616
CACGTCT93750.038.1189514
CACACGT93150.037.87027412
ATCGGAA65450.037.598082
CGGAAGA98100.037.5936664
TCTCGTA87950.037.2358141
GCACACG97900.036.154911
GACCAAT91600.035.96749535
ACTGACC104700.035.8991232
ATCTCGT94150.034.7132340
TGACCAA107250.034.4100934
TCGGAAG75200.034.18553
GAACTCC109950.033.60787621
Produced by FastQC (version 0.11.2)