FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M21M42-NEBNext5_ACAGTG-_AC8KUAANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext5_ACAGTG-_AC8KUAANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9682520
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG245700.25375625353730225Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT100500.040.77109511
CGTCGTG102500.040.3605412
TCGTGTA109450.040.14573314
CGTGTAG109100.039.80577515
GATCGGA114350.039.6548921
AGCGTCG101650.039.4878310
GTAGGGA105900.039.1248818
GTCGTGT106800.038.87606413
ATCGGAA116600.038.482062
AGAGCGT107100.038.2239278
GATCTCG68850.038.0204334
GAGCGTC105150.038.0061159
TGTAGGG112750.037.83884417
ATCTCGG71750.037.72183235
GTAGATC77500.037.1895631
TAGGGAA111350.037.02766419
TCGGAAG124650.036.7020343
AAGAGCG110550.036.553627
TCTCGGT72100.036.5250436
GTGTAGG117100.036.3794116
Produced by FastQC (version 0.11.2)