FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M21M42-NEBNext6_GCCAAT-_AC8KUAANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext6_GCCAAT-_AC8KUAANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16203722
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC835780.5157950747365327TruSeq Adapter, Index 6 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400230.24699880681734726No Hit
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC246130.1518971999149331TruSeq Adapter, Index 6 (98% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA199150.12290386122398297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCAA198200.040.8470932
GTCACGC205300.040.82650829
CGTATGC201400.040.8205444
CGCCAAT198850.040.4923633
TCGTATG202600.040.49191743
TCACGCC206600.040.0380930
AGTCACG212250.039.85369528
CTCGTAT201100.039.80964342
ACACGTC198350.039.263113
ACGTCTG196000.039.21734615
CGTCTGA193400.039.10701816
TATCTCG183500.038.97757339
CACGCCA209550.038.76072731
GATCGGA136050.038.749671
TCTCGTA189100.038.2768941
CGGAAGA212900.038.2381364
CACGTCT207200.038.00063314
ATCGGAA142300.037.7681352
CACACGT206500.037.39541212
GCCAATA215100.037.3718534
Produced by FastQC (version 0.11.2)