FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M21M42-NEBNext8_ACTTGA-_AC8KUAANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext8_ACTTGA-_AC8KUAANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11716980
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA604380.5158155087744453No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387930.33108360686798133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT139250.041.51866511
CGTCGTG141850.041.17395412
CGTGTAG149500.039.9991615
TCGTGTA151500.039.8960714
GTCGTGT146350.039.88931713
GTAGGGA143400.039.68494818
GATCTCG89350.039.63508234
AGCGTCG143850.039.54976310
AGAGCGT147250.039.514088
GAGCGTC145150.039.3925069
GATCGGA160850.039.1311951
ATCTCGG93100.038.87396635
TGTAGGG151550.038.8121717
ATCGGAA162550.038.3982542
GTGTAGG154600.037.6647916
GTCGCCG74900.037.6631644
TCTCGGT93700.037.58563636
GAGTGTA135000.037.3358427
TAGGGAA150300.037.33162319
AAGAGCG152450.037.316057
Produced by FastQC (version 0.11.2)