FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M21M42-NEBNext9_GATCAG-_AC8KUAANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext9_GATCAG-_AC8KUAANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10212050
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC411250.40271052335231416TruSeq Adapter, Index 9 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343970.3368275713495331No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA319980.31333571613926686No Hit
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC118770.11630377837946347TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG99400.040.18191543
ACGATCA99850.040.0230532
CGTATGC101050.039.8958144
CGATCAG102750.038.82903733
AGTCACG105600.038.7819528
TCACGAT104000.038.72204230
CTCGTAT98250.038.3465242
GTCACGA106550.038.08450329
CACGATC105150.038.02663431
ACACGTC103050.037.28496613
ACGTCTG102050.036.87358515
CGTCTGA100750.036.58446516
TCTCGTA92400.036.1085941
CGGAAGA110300.035.8675354
CACACGT108550.035.1956612
CACGTCT110550.035.17371414
ATCGGAA78750.034.095592
GATCGGA77050.034.08111
GCACACG113050.033.81969511
GATCTCG99000.033.5451839
Produced by FastQC (version 0.11.2)