FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M21M42-NEBNext9_GATCAG-_AC8KUAANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext9_GATCAG-_AC8KUAANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10212050
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA371310.3635998648655265No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192560.18856155228382157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT78750.040.4105211
CGTCGTG82250.039.1439412
TCGTGTA86000.038.56810414
CGTGTAG84800.038.5627315
AGCGTCG81750.038.20439510
GATCGGA91400.038.123921
GTCGTGT84500.037.78655213
GTAGGGA83300.037.53364618
GAGCGTC84600.037.021369
AGAGCGT85850.036.9868858
TGTAGGG88100.036.71074717
ATCGGAA94850.036.4483452
ATCTCGG55350.035.9146135
GATCTCG53150.035.89758334
TCTCGGT54650.035.48344436
AAGAGCG88000.035.2832877
GTAGATC61700.035.11166431
TAGGGAA89600.034.84025619
GTGTAGG93200.034.3493616
TCGGAAG104050.034.0543483
Produced by FastQC (version 0.11.2)