FastQCFastQC Report
Wed 4 May 2016
G128-M11_GGCTAC_BC8GTYANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M11_GGCTAC_BC8GTYANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10717620
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC3714013.465330922350298TruSeq Adapter, Index 11 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT659670.6155004562580125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA485100.043.5140951
CGTATGC486150.042.9940544
ATCGGAA490850.042.993312
ACACGTC500700.042.7517213
ACGGCTA489250.042.72609332
TCGTATG490750.042.66045843
CTCGTAT477400.042.6260342
ACGTCTG502900.042.59808315
CGTCTGA501450.042.59647816
CACACGT498150.042.5793312
CGGCTAC489750.042.4119433
CACGTCT506250.042.33041814
CGGAAGA499150.042.178114
TCTCGTA447600.042.07518841
TCACGGC493000.042.0615330
CACGGCT493300.042.0185131
AGTCACG503250.041.60802528
GTCACGG499550.041.57149529
TCGGAAG514350.041.4006083
ATCTCGT455150.041.29502540