Basic Statistics
Measure | Value |
---|---|
Filename | G128-M11_GGCTAC_BC8GTYANXX_L001_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 10717620 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 420247 | 3.921085091652811 | Illumina Single End PCR Primer 1 (100% over 50bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 57638 | 0.5377873072566484 | No Hit |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 14864 | 0.13868750711445266 | No Hit |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | 12307 | 0.11482959836232298 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCGTGTA | 48960 | 0.0 | 42.826714 | 14 |
GATCTCG | 50035 | 0.0 | 42.81032 | 34 |
ATCTCGG | 50065 | 0.0 | 42.7619 | 35 |
GTAGATC | 50275 | 0.0 | 42.648518 | 31 |
TCTCGGT | 50250 | 0.0 | 42.64784 | 36 |
GATCGGA | 50030 | 0.0 | 42.57724 | 1 |
GTCGTGT | 49735 | 0.0 | 42.47203 | 13 |
GTGGTCG | 49865 | 0.0 | 42.448635 | 41 |
ATCGGAA | 49925 | 0.0 | 42.437492 | 2 |
CGTGTAG | 49725 | 0.0 | 42.396713 | 15 |
GTAGGGA | 49710 | 0.0 | 42.31234 | 18 |
AGAGCGT | 50510 | 0.0 | 42.30102 | 8 |
GAGTGTA | 50660 | 0.0 | 42.293423 | 27 |
CGTCGTG | 50185 | 0.0 | 42.24999 | 12 |
CTCGGTG | 50675 | 0.0 | 42.208492 | 37 |
TCGGTGG | 50970 | 0.0 | 42.111504 | 38 |
GGTCGCC | 50110 | 0.0 | 42.09324 | 43 |
TAGATCT | 50880 | 0.0 | 42.062206 | 32 |
AGTGTAG | 51030 | 0.0 | 41.979126 | 28 |
GTGTAGG | 50430 | 0.0 | 41.943413 | 16 |