FastQCFastQC Report
Wed 4 May 2016
G128-M11_GGCTAC_BC8GTYANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M11_GGCTAC_BC8GTYANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10717620
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4202473.921085091652811Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA576380.5377873072566484No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148640.13868750711445266No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN123070.11482959836232298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA489600.042.82671414
GATCTCG500350.042.8103234
ATCTCGG500650.042.761935
GTAGATC502750.042.64851831
TCTCGGT502500.042.6478436
GATCGGA500300.042.577241
GTCGTGT497350.042.4720313
GTGGTCG498650.042.44863541
ATCGGAA499250.042.4374922
CGTGTAG497250.042.39671315
GTAGGGA497100.042.3123418
AGAGCGT505100.042.301028
GAGTGTA506600.042.29342327
CGTCGTG501850.042.2499912
CTCGGTG506750.042.20849237
TCGGTGG509700.042.11150438
GGTCGCC501100.042.0932443
TAGATCT508800.042.06220632
AGTGTAG510300.041.97912628
GTGTAGG504300.041.94341316