FastQCFastQC Report
Wed 4 May 2016
G128-M14_ATGTCA_BC8GTYANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M14_ATGTCA_BC8GTYANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17945969
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT400840.22335935161818232TruSeq Adapter, Index 15 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220460.12284652893360062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA63100.036.5146331
CGTCTGA69200.033.6529716
CTCGTAT63150.033.53772744
ACGTCTG69450.033.4993215
ATCGGAA68650.033.3187032
ACACGTC70450.033.0823713
AATCTCG60600.031.7862241
CACGTCT78450.029.65536314
TCTCGTA66450.029.60213943
CACACGT78850.029.40714312
CGGAAGA83200.028.3096734
TCGGAAG89200.026.508633
GCACACG88850.026.07248711
ATCTCGT80350.024.4555642
GAACTCC103650.022.59217821
ACATGTC100150.022.58293232
GAATCTC87900.022.21520440
TCTGAAC112550.020.8856318
CACATGT109450.020.54361731
ATGTCAG110200.020.44264634
Produced by FastQC (version 0.11.2)