FastQCFastQC Report
Wed 4 May 2016
G128-M14_ATGTCA_BC8GTYANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M14_ATGTCA_BC8GTYANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17945969
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG486220.270935495319311Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN229840.12807332944796684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG75500.033.7925134
GTAGATC78300.032.89583631
GTCGCCG77000.032.65500644
TCTCGGT80000.032.49679636
ATCTCGG78350.032.45128335
GCGTCGT79000.031.91673711
TCGTGTA77800.031.38796414
CGTGTAG81950.030.57501215
CGTCGTG82800.030.5294612
GTGGTCG84000.030.05924441
TGGTCGC85050.029.68756342
GTAGGGA83700.029.64713918
GTCGTGT84750.029.64430813
GGTCGCC85650.029.5065143
AGCGTCG87700.028.97786710
GAGTGTA89300.028.47559427
CTCGGTG91550.028.22904637
GAGCGTC91600.028.1062139
CGGTGGT90450.028.0127539
AGAGCGT91650.027.8048658
Produced by FastQC (version 0.11.2)