Basic Statistics
Measure | Value |
---|---|
Filename | G128-M2_CGATGT_BC8GTYANXX_L001_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 13755278 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC | 403529 | 2.933630276320115 | TruSeq Adapter, Index 2 (100% over 50bp) |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 83729 | 0.6087045278183401 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 53060 | 0.0 | 43.360332 | 1 |
ATCGGAA | 53480 | 0.0 | 42.988266 | 2 |
CGTATGC | 53080 | 0.0 | 42.808456 | 44 |
ACACGTC | 54340 | 0.0 | 42.771984 | 13 |
ACGTCTG | 54555 | 0.0 | 42.73364 | 15 |
CGTCTGA | 54450 | 0.0 | 42.680603 | 16 |
CTCGTAT | 52290 | 0.0 | 42.554764 | 42 |
CCGATGT | 52450 | 0.0 | 42.533726 | 33 |
TCACCGA | 52670 | 0.0 | 42.523464 | 30 |
TCGTATG | 53635 | 0.0 | 42.504482 | 43 |
CGATGTA | 52330 | 0.0 | 42.48959 | 34 |
CACGTCT | 54970 | 0.0 | 42.44148 | 14 |
CACCGAT | 52570 | 0.0 | 42.41261 | 31 |
TCGGAAG | 54660 | 0.0 | 42.31758 | 3 |
CACACGT | 54520 | 0.0 | 42.30376 | 12 |
ACCGATG | 53115 | 0.0 | 42.30036 | 32 |
TATCTCG | 48425 | 0.0 | 42.263504 | 39 |
CGGAAGA | 54130 | 0.0 | 42.232018 | 4 |
TCTCGTA | 48995 | 0.0 | 42.133762 | 41 |
GTCACCG | 53545 | 0.0 | 42.127445 | 29 |