FastQCFastQC Report
Wed 4 May 2016
G128-M2_CGATGT_BC8GTYANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M2_CGATGT_BC8GTYANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13755278
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC4035292.933630276320115TruSeq Adapter, Index 2 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT837290.6087045278183401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA530600.043.3603321
ATCGGAA534800.042.9882662
CGTATGC530800.042.80845644
ACACGTC543400.042.77198413
ACGTCTG545550.042.7336415
CGTCTGA544500.042.68060316
CTCGTAT522900.042.55476442
CCGATGT524500.042.53372633
TCACCGA526700.042.52346430
TCGTATG536350.042.50448243
CGATGTA523300.042.4895934
CACGTCT549700.042.4414814
CACCGAT525700.042.4126131
TCGGAAG546600.042.317583
CACACGT545200.042.3037612
ACCGATG531150.042.3003632
TATCTCG484250.042.26350439
CGGAAGA541300.042.2320184
TCTCGTA489950.042.13376241
GTCACCG535450.042.12744529