Basic Statistics
Measure | Value |
---|---|
Filename | G128-M2_CGATGT_BC8GTYANXX_L001_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 13755278 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 475461 | 3.4565713611895013 | Illumina Single End PCR Primer 1 (100% over 50bp) |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 51652 | 0.37550676911073694 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 43206 | 0.3141048839579978 | No Hit |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | 17069 | 0.12409054909686304 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCGTGTA | 54030 | 0.0 | 42.79824 | 14 |
GATCGGA | 55030 | 0.0 | 42.675423 | 1 |
ATCTCGG | 55000 | 0.0 | 42.67099 | 35 |
GATCTCG | 55025 | 0.0 | 42.66744 | 34 |
TCTCGGT | 55180 | 0.0 | 42.63144 | 36 |
GTGGTCG | 55025 | 0.0 | 42.500492 | 41 |
ATCGGAA | 55025 | 0.0 | 42.495094 | 2 |
CGTGTAG | 54640 | 0.0 | 42.49048 | 15 |
GTCGTGT | 54855 | 0.0 | 42.45567 | 13 |
AGAGCGT | 55450 | 0.0 | 42.43473 | 8 |
GGTCGCC | 54860 | 0.0 | 42.432804 | 43 |
CTCGGTG | 55515 | 0.0 | 42.34326 | 37 |
CGTCGTG | 55135 | 0.0 | 42.328922 | 12 |
GTAGATC | 55705 | 0.0 | 42.274216 | 31 |
GCGTCGT | 55190 | 0.0 | 42.270504 | 11 |
TCGGTGG | 55905 | 0.0 | 42.16589 | 38 |
GTCGCCG | 55435 | 0.0 | 42.053898 | 44 |
TGGTCGC | 55375 | 0.0 | 42.049164 | 42 |
CGGTGGT | 55960 | 0.0 | 42.022263 | 39 |
GAGCGTC | 56000 | 0.0 | 42.01699 | 9 |