FastQCFastQC Report
Wed 4 May 2016
G128-M2_CGATGT_BC8GTYANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M2_CGATGT_BC8GTYANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13755278
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4754613.4565713611895013Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT516520.37550676911073694No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA432060.3141048839579978No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN170690.12409054909686304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA540300.042.7982414
GATCGGA550300.042.6754231
ATCTCGG550000.042.6709935
GATCTCG550250.042.6674434
TCTCGGT551800.042.6314436
GTGGTCG550250.042.50049241
ATCGGAA550250.042.4950942
CGTGTAG546400.042.4904815
GTCGTGT548550.042.4556713
AGAGCGT554500.042.434738
GGTCGCC548600.042.43280443
CTCGGTG555150.042.3432637
CGTCGTG551350.042.32892212
GTAGATC557050.042.27421631
GCGTCGT551900.042.27050411
TCGGTGG559050.042.1658938
GTCGCCG554350.042.05389844
TGGTCGC553750.042.04916442
CGGTGGT559600.042.02226339
GAGCGTC560000.042.016999