FastQCFastQC Report
Wed 4 May 2016
G128-M4_TGACCA_BC8GTYANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M4_TGACCA_BC8GTYANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10120170
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3780833.7359352659095646TruSeq Adapter, Index 4 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700060.6917472730201172No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT689560.6813719532379396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA497550.043.684231
ATCGGAA503000.043.198612
CGTATGC498300.043.07180844
ACACGTC513250.043.01515613
TCGGAAG508400.043.002733
CGTCTGA511050.042.98276516
ACGTCTG514150.042.94431315
CGGAAGA502950.042.9076964
CACGTCT518050.042.70464714
TCGTATG503050.042.6991243
CTCGTAT480750.042.69070442
CACACGT512700.042.61385312
TCTCGTA448350.042.5292641
ATCTCGT452050.041.9776740
ACTGACC511600.041.87468332
GACCAAT466200.041.85685735
AATCTCG453200.041.67405739
GAACTCC525250.041.58299321
ACCAATC453050.041.56336236
TGACCAA513500.041.2633334
Produced by FastQC (version 0.11.2)