G128-M4_TGACCA_BC8GTYANXX_L001_001.R2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	G128-M4_TGACCA_BC8GTYANXX_L001_001.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10120170
Sequences flagged as poor quality	0
Sequence length	50
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	459530	4.540733999527676	Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	128594	1.270670354351755	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48949	0.4836776457312476	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	11105	0.10973135826769709	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TCGTGTA	51435	43.081436	14
GATCTCG	52065	43.050194	34
ATCGGAA	51830	43.028263	2
GATCGGA	52035	43.02395	1
ATCTCGG	52160	42.984436	35
AGAGCGT	52205	42.91283	8
CGTGTAG	51825	42.88777	15
GGTCGCC	51695	42.83649	43
TCTCGGT	52380	42.812294	36
GTGGTCG	51910	42.810547	41
GTCGTGT	52040	42.80465	13
CGTCGTG	52240	42.69194	12
CTCGGTG	52445	42.680187	37
GCGTCGT	52290	42.662495	11
AAGAGCG	52645	42.6094	7
TGGTCGC	52080	42.580223	42
TCGGTGG	52820	42.531456	38
GTAGATC	52785	42.496563	31
TCGGAAG	52930	42.45365	3
CGGTGGT	52775	42.429768	39