FastQCFastQC Report
Wed 4 May 2016
G128-M5A_ACAGTG_BC8GTYANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M5A_ACAGTG_BC8GTYANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12138023
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC3413602.8123196009762053TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA456050.043.320541
ATCGGAA459750.042.9192922
CGTATGC460250.042.63711544
ACACGTC472350.042.46320313
ACGTCTG474000.042.3525715
CTCGTAT442850.042.30460442
TCGTATG464150.042.2746843
CGTCTGA473000.042.26788316
CACACGT473800.042.06788312
CACGTCT478550.041.98388314
TCTCGTA408100.041.8457241
AGTGATC406900.041.49122636
TCGGAAG479600.041.4759983
CGGAAGA474150.041.443174
ATCTCGT410800.041.41612240
GCACACG480400.041.3981711
GATCTCG411650.041.15428539
GAACTCC496300.040.0924521
GTCTGAA502000.039.93935417
CTGAACT502350.039.8339919
Produced by FastQC (version 0.11.2)