FastQCFastQC Report
Wed 4 May 2016
G128-M6_GCCAAT_BC8GTYANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M6_GCCAAT_BC8GTYANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12121107
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC4096463.3796088096574013TruSeq Adapter, Index 6 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT745420.6149768333865876No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA203900.16821895887892088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA525550.043.536881
ATCGGAA531600.042.9862672
CGTCTGA539700.042.80463416
ACACGTC540050.042.80293713
CGTATGC526100.042.80192644
ACGTCTG541500.042.74128315
CGCCAAT522100.042.67058633
CACACGT536850.042.59459312
GTCACGC526400.042.5672129
TCGGAAG540950.042.54043
ACGCCAA527500.042.5300832
TCGTATG530450.042.45863743
CTCGTAT521100.042.42576642
CACGTCT545400.042.40567814
CGGAAGA538000.042.278784
TATCTCG480550.042.25163339
AGTCACG534150.042.2063628
TCTCGTA488800.041.88876341
TCACGCC536450.041.67166530
GCCAATA532100.041.56442334