Basic Statistics
Measure | Value |
---|---|
Filename | G128-M6_GCCAAT_BC8GTYANXX_L001_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 12121107 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC | 409646 | 3.3796088096574013 | TruSeq Adapter, Index 6 (100% over 50bp) |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 74542 | 0.6149768333865876 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 20390 | 0.16821895887892088 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 52555 | 0.0 | 43.53688 | 1 |
ATCGGAA | 53160 | 0.0 | 42.986267 | 2 |
CGTCTGA | 53970 | 0.0 | 42.804634 | 16 |
ACACGTC | 54005 | 0.0 | 42.802937 | 13 |
CGTATGC | 52610 | 0.0 | 42.801926 | 44 |
ACGTCTG | 54150 | 0.0 | 42.741283 | 15 |
CGCCAAT | 52210 | 0.0 | 42.670586 | 33 |
CACACGT | 53685 | 0.0 | 42.594593 | 12 |
GTCACGC | 52640 | 0.0 | 42.56721 | 29 |
TCGGAAG | 54095 | 0.0 | 42.5404 | 3 |
ACGCCAA | 52750 | 0.0 | 42.53008 | 32 |
TCGTATG | 53045 | 0.0 | 42.458637 | 43 |
CTCGTAT | 52110 | 0.0 | 42.425766 | 42 |
CACGTCT | 54540 | 0.0 | 42.405678 | 14 |
CGGAAGA | 53800 | 0.0 | 42.27878 | 4 |
TATCTCG | 48055 | 0.0 | 42.251633 | 39 |
AGTCACG | 53415 | 0.0 | 42.20636 | 28 |
TCTCGTA | 48880 | 0.0 | 41.888763 | 41 |
TCACGCC | 53645 | 0.0 | 41.671665 | 30 |
GCCAATA | 53210 | 0.0 | 41.564423 | 34 |