FastQCFastQC Report
Wed 4 May 2016
G128-M6_GCCAAT_BC8GTYANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M6_GCCAAT_BC8GTYANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12121107
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4823533.979446761752041Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610200.5034193659044508No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT403200.3326428848454188No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN137960.11381798708649302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA548400.042.7898331
GATCTCG545900.042.77559334
TCGTGTA538450.042.75645414
ATCTCGG547750.042.63164535
ATCGGAA547750.042.6155552
CGTGTAG542550.042.5955415
TCTCGGT549500.042.5839336
GTGGTCG547150.042.53805541
AGAGCGT548850.042.5329938
GTCGTGT546400.042.47711613
GTAGATC551750.042.4699531
CGTCGTG546950.042.43107612
CTCGGTG552200.042.28428337
GCGTCGT548950.042.275811
TGGTCGC548650.042.27185442
GTAGGGA547050.042.22976318
GGTCGCC548850.042.1977443
TCGGTGG554750.042.1892138
AAGAGCG555500.042.1113667
CGGTGGT554300.042.10407339