Basic Statistics
Measure | Value |
---|---|
Filename | G128-M6_GCCAAT_BC8GTYANXX_L001_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 12121107 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 482353 | 3.979446761752041 | Illumina Single End PCR Primer 1 (100% over 50bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 61020 | 0.5034193659044508 | No Hit |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 40320 | 0.3326428848454188 | No Hit |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | 13796 | 0.11381798708649302 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 54840 | 0.0 | 42.789833 | 1 |
GATCTCG | 54590 | 0.0 | 42.775593 | 34 |
TCGTGTA | 53845 | 0.0 | 42.756454 | 14 |
ATCTCGG | 54775 | 0.0 | 42.631645 | 35 |
ATCGGAA | 54775 | 0.0 | 42.615555 | 2 |
CGTGTAG | 54255 | 0.0 | 42.59554 | 15 |
TCTCGGT | 54950 | 0.0 | 42.58393 | 36 |
GTGGTCG | 54715 | 0.0 | 42.538055 | 41 |
AGAGCGT | 54885 | 0.0 | 42.532993 | 8 |
GTCGTGT | 54640 | 0.0 | 42.477116 | 13 |
GTAGATC | 55175 | 0.0 | 42.46995 | 31 |
CGTCGTG | 54695 | 0.0 | 42.431076 | 12 |
CTCGGTG | 55220 | 0.0 | 42.284283 | 37 |
GCGTCGT | 54895 | 0.0 | 42.2758 | 11 |
TGGTCGC | 54865 | 0.0 | 42.271854 | 42 |
GTAGGGA | 54705 | 0.0 | 42.229763 | 18 |
GGTCGCC | 54885 | 0.0 | 42.19774 | 43 |
TCGGTGG | 55475 | 0.0 | 42.18921 | 38 |
AAGAGCG | 55550 | 0.0 | 42.111366 | 7 |
CGGTGGT | 55430 | 0.0 | 42.104073 | 39 |