FastQCFastQC Report
Wed 4 May 2016
G128-M7_CAGATC_BC8GTYANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M7_CAGATC_BC8GTYANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12600419
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC7034825.58300481912546TruSeq Adapter, Index 7 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940450.7463640693218218No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690310.5478468612829462No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAACTCGTATGC173370.13759066265970996TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCACCTCGTATGC157680.12513869578464018TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAGCTCGTATGC126510.10040142315902352TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA910950.043.6905361
ATCGGAA916850.043.3643532
CGTATGC908300.043.20943544
CGTCTGA933350.043.03998616
ACACGTC935600.043.03827313
ACGTCTG937800.043.03821615
TCGTATG915850.042.9138143
CTCGTAT890300.042.8664942
CACGTCT942750.042.8310714
CACACGT931450.042.79891612
CGGAAGA919650.042.7613374
TCGGAAG936400.042.5613633
TCTCGTA827200.042.550341
CATCTCG825150.042.16343339
ATCTCGT832600.042.160840
GAACTCC949950.042.0897521
ACCAGAT922850.042.04509432
GCACACG948850.041.9764711
CTGAACT960200.041.9317719
GATCATC829050.041.89476836