Basic Statistics
Measure | Value |
---|---|
Filename | G128-M7_CAGATC_BC8GTYANXX_L001_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 12600419 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC | 703482 | 5.58300481912546 | TruSeq Adapter, Index 7 (100% over 50bp) |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 94045 | 0.7463640693218218 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 69031 | 0.5478468612829462 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAACTCGTATGC | 17337 | 0.13759066265970996 | TruSeq Adapter, Index 7 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCACCTCGTATGC | 15768 | 0.12513869578464018 | TruSeq Adapter, Index 7 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAGCTCGTATGC | 12651 | 0.10040142315902352 | TruSeq Adapter, Index 7 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 91095 | 0.0 | 43.690536 | 1 |
ATCGGAA | 91685 | 0.0 | 43.364353 | 2 |
CGTATGC | 90830 | 0.0 | 43.209435 | 44 |
CGTCTGA | 93335 | 0.0 | 43.039986 | 16 |
ACACGTC | 93560 | 0.0 | 43.038273 | 13 |
ACGTCTG | 93780 | 0.0 | 43.038216 | 15 |
TCGTATG | 91585 | 0.0 | 42.91381 | 43 |
CTCGTAT | 89030 | 0.0 | 42.86649 | 42 |
CACGTCT | 94275 | 0.0 | 42.83107 | 14 |
CACACGT | 93145 | 0.0 | 42.798916 | 12 |
CGGAAGA | 91965 | 0.0 | 42.761337 | 4 |
TCGGAAG | 93640 | 0.0 | 42.561363 | 3 |
TCTCGTA | 82720 | 0.0 | 42.5503 | 41 |
CATCTCG | 82515 | 0.0 | 42.163433 | 39 |
ATCTCGT | 83260 | 0.0 | 42.1608 | 40 |
GAACTCC | 94995 | 0.0 | 42.08975 | 21 |
ACCAGAT | 92285 | 0.0 | 42.045094 | 32 |
GCACACG | 94885 | 0.0 | 41.97647 | 11 |
CTGAACT | 96020 | 0.0 | 41.93177 | 19 |
GATCATC | 82905 | 0.0 | 41.894768 | 36 |