Basic Statistics
Measure | Value |
---|---|
Filename | G128-M7_CAGATC_BC8GTYANXX_L001_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 12600419 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 842892 | 6.689396598636918 | Illumina Single End PCR Primer 1 (100% over 50bp) |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 222756 | 1.767845974010864 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 60682 | 0.48158715991904716 | No Hit |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | 15112 | 0.11993251970430507 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCGTGTA | 92225 | 0.0 | 43.42307 | 14 |
GATCGGA | 93600 | 0.0 | 43.344074 | 1 |
GATCTCG | 93455 | 0.0 | 43.29333 | 34 |
CGTGTAG | 92815 | 0.0 | 43.264698 | 15 |
ATCTCGG | 93595 | 0.0 | 43.23831 | 35 |
GTCGTGT | 93175 | 0.0 | 43.238045 | 13 |
ATCGGAA | 93625 | 0.0 | 43.219643 | 2 |
AGAGCGT | 93885 | 0.0 | 43.202057 | 8 |
GTGGTCG | 93475 | 0.0 | 43.161247 | 41 |
TCTCGGT | 94065 | 0.0 | 43.125153 | 36 |
CGTCGTG | 93465 | 0.0 | 43.104572 | 12 |
GCGTCGT | 93445 | 0.0 | 43.094837 | 11 |
GTAGATC | 94220 | 0.0 | 43.03298 | 31 |
GGTCGCC | 93415 | 0.0 | 42.985497 | 43 |
TGGTCGC | 93490 | 0.0 | 42.982243 | 42 |
TCGGTGG | 94760 | 0.0 | 42.955593 | 38 |
GTAGGGA | 93560 | 0.0 | 42.93917 | 18 |
CGGTGGT | 94430 | 0.0 | 42.91972 | 39 |
AAGAGCG | 94725 | 0.0 | 42.89615 | 7 |
CTCGGTG | 94550 | 0.0 | 42.878517 | 37 |