FastQCFastQC Report
Wed 4 May 2016
G128-M7_CAGATC_BC8GTYANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M7_CAGATC_BC8GTYANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12600419
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG8428926.689396598636918Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2227561.767845974010864No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA606820.48158715991904716No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN151120.11993251970430507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA922250.043.4230714
GATCGGA936000.043.3440741
GATCTCG934550.043.2933334
CGTGTAG928150.043.26469815
ATCTCGG935950.043.2383135
GTCGTGT931750.043.23804513
ATCGGAA936250.043.2196432
AGAGCGT938850.043.2020578
GTGGTCG934750.043.16124741
TCTCGGT940650.043.12515336
CGTCGTG934650.043.10457212
GCGTCGT934450.043.09483711
GTAGATC942200.043.0329831
GGTCGCC934150.042.98549743
TGGTCGC934900.042.98224342
TCGGTGG947600.042.95559338
GTAGGGA935600.042.9391718
CGGTGGT944300.042.9197239
AAGAGCG947250.042.896157
CTCGGTG945500.042.87851737