FastQCFastQC Report
Wed 4 May 2016
G128-M9_GATCAG_BC8GTYANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M9_GATCAG_BC8GTYANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17156077
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1706420.9946446381652402TruSeq Adapter, Index 9 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT668210.3894888091257692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA231650.042.519391
CGTATGC235300.041.5267344
TCGTATG237650.041.2837843
CTCGTAT227650.041.16757642
ATCGGAA240000.041.090282
CGTCTGA242050.040.9640516
ACACGTC243500.040.93041213
ACGTCTG244450.040.85658315
ACGATCA238750.040.67553332
TCACGAT239100.040.5238630
CGATCAG240400.040.22176733
GTCACGA243550.039.9183529
CACGTCT251550.039.8425714
CACGATC242500.039.7656331
CACACGT249200.039.54093612
TCGGAAG254850.039.0712663
TCTCGTA218400.039.0492241
AGTCACG252100.038.88877528
CGGAAGA255450.038.525514
GATCTCG220250.038.29266439
Produced by FastQC (version 0.11.2)