FastQCFastQC Report
Wed 4 May 2016
G131-M10_CGTACG_BC8GTYANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M10_CGTACG_BC8GTYANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8416513
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG932441.1078697317998558Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT796120.9459024182580126No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120700.14340855886517376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG104950.041.19806734
ATCTCGG105350.040.95740535
TCTCGGT107150.040.31041736
TCGTGTA105900.040.2000114
GCGTCGT108350.039.8612211
CGTCGTG108450.039.76336712
GTCGTGT108700.039.50951813
CGTGTAG108300.039.50594715
GTAGATC110550.039.392731
AGCGTCG109850.039.33835210
TGGTCGC110400.039.1466942
GTCGCCG110650.038.90246644
GATCGGA111450.038.8489271
ATCGGAA111350.038.763762
GTGGTCG112000.038.6856441
GTAGGGA110350.038.60980618
AGAGCGT112200.038.597918
GAGCGTC112450.038.507299
CTCGGTG113700.038.0269137
CGGTGGT114400.037.91069839
Produced by FastQC (version 0.11.2)