FastQCFastQC Report
Wed 4 May 2016
G131-M11_GAGTGG_BC8GTYANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M11_GAGTGG_BC8GTYANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7857957
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT461180.5868955505864947TruSeq Adapter, Index 7 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170850.21742292557722065No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124060.1578781864039215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA67300.041.5894621
ATCGGAA68450.040.6264572
ACGAGTG65450.040.62602232
CGTCTGA68900.040.09353616
CTCGTAT63300.040.07050344
ACACGTC69100.039.71885313
CACGAGT67000.039.69274531
ACGTCTG70000.039.68348315
CGAGTGG67600.039.4127533
TCACGAG68000.039.23431430
AGTCACG69550.039.18297628
GTCACGA69300.038.5504529
CACGTCT72600.038.412814
TATCTCG62150.038.05561441
TCGGAAG73550.037.9888273
CGGAAGA73350.037.7656444
CACACGT73400.037.5756612
TCTCGTA63100.037.51750643
ATCTCGT64600.036.8164942
GCACACG78500.034.96776211
Produced by FastQC (version 0.11.2)