FastQCFastQC Report
Wed 4 May 2016
G131-M12_ACTGAT_BC8GTYANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M12_ACTGAT_BC8GTYANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16945353
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT396030.23371009149234012TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA65800.036.3903731
TATCTCG59050.035.58906641
CTCGTAT64250.034.34905244
ACACGTC70800.033.534513
ATCGGAA71950.033.273872
CGTCTGA72200.033.03846416
ACGTCTG72350.032.88386515
TCTCGTA65900.031.65530443
CACGTCT76550.031.36417614
CACACGT79400.030.04709812
ATCTCGT72850.028.72662742
CGGAAGA87900.028.1152424
ATATATC75850.027.71363438
GCACACG87750.027.21452711
GATATAT79500.027.05228837
TCGGAAG92000.026.6919043
TGATATA88300.026.37616236
ATATCTC81050.025.94493740
ACTGATA92450.025.50163834
CTGATAT93200.025.10741835
Produced by FastQC (version 0.11.2)