FastQCFastQC Report
Wed 4 May 2016
G131-M12_ACTGAT_BC8GTYANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M12_ACTGAT_BC8GTYANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16945353
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG530260.31292354901075237Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG73700.033.7803434
GTAGATC74950.033.4539831
GCGTCGT75700.033.01724211
TCGTGTA75600.032.6809414
ATCTCGG77650.032.51474435
TCTCGGT77550.032.44333336
CGTGTAG76950.032.27531415
CGTCGTG77350.032.25594712
GTCGCCG81600.030.70232644
GTCGTGT82900.030.06948913
AGCGTCG84050.029.97342710
TGGTCGC83250.029.9593142
GTGGTCG85800.029.22263141
GTAGGGA85300.029.16540118
TGTAGGG88050.028.50457217
GAGCGTC89700.028.3313439
GAGTGTA88800.028.31512327
TAGATCT89000.028.22110432
GGTCGCC88500.028.20716943
GATCGGA89000.028.1060561
Produced by FastQC (version 0.11.2)