FastQCFastQC Report
Wed 4 May 2016
G131-M13_ATTCCT_BC8GTYANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M13_ATTCCT_BC8GTYANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15306100
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182620.11931190832413222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA18000.028.4376011
TATCTCG18950.021.92440641
ATCGGAA23300.021.4935872
CGTCTGA23750.021.25445416
CTCGTAT23600.020.3972644
ACGTCTG26600.019.37780215
ACACGTC27200.019.27182413
CACACGT31550.016.25487712
CACGTCT33100.016.10340714
TCTCGTA25300.015.98631643
CGGAAGA36500.015.8289654
ATCTCGT30050.013.8988942
GCACACG41750.012.6008111
TCGGAAG45050.012.3848353
GTCGGTT24050.011.6924191
AATCCGA18550.010.32254624
ATCCGAA19050.010.16914825
AAATCCG19300.09.82762923
GGTACGA14150.09.7807864
TACGAGG14650.09.6093386
Produced by FastQC (version 0.11.2)