FastQCFastQC Report
Wed 4 May 2016
G131-M13_ATTCCT_BC8GTYANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M13_ATTCCT_BC8GTYANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15306100
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG26900.021.58403634
TCTCGGT28400.021.14105836
GCGTCGT27200.020.86998711
TCGTGTA27950.019.91525814
ATCTCGG29550.019.87136535
GTAGATC30150.019.33211131
CGTGTAG29100.019.20100815
CGTCGTG30650.019.0230912
GTCGCCG30400.018.73988244
AGCGTCG31850.018.58293210
GTCGTGT32950.017.56139213
GTGGTCG35750.016.4880641
GATCGGA35150.016.4759391
TGGTCGC35800.016.34226442
GTAGGGA36100.015.59872218
GGTCGCC37850.015.34103143
GAGTGTA38200.015.31778227
TGTAGGG37750.015.2666417
TAGATCT38600.015.21369732
CGGTGGT39700.015.17957139
Produced by FastQC (version 0.11.2)