FastQCFastQC Report
Wed 4 May 2016
G131-M14_AGTCAA_BC8GTYANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M14_AGTCAA_BC8GTYANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10296694
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT423310.4111125376747138TruSeq Adapter, Index 13 (97% over 40bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA407310.3955735695360084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA66450.040.398371
ATCGGAA68600.038.9658742
ACGTCTG70550.037.37740315
ACACGTC70150.037.24145513
AATCTCG62350.036.59778241
CGTCTGA72350.036.50570716
CTCGTAT67400.036.2664544
CACGTCT74950.035.2415714
CGGAAGA75400.035.1941534
CACACGT74900.034.96901712
TCTCGTA67550.033.6808943
AGTCAAC77400.032.89830434
TCGGAAG83100.032.4592553
GCACACG81100.032.37898611
ATCTCGT72950.031.4301642
CAGTCAA85550.030.16519233
GTCAACA86500.029.53944435
GAACTCC88400.029.33130821
TCTGAAC92150.028.42431818
CTGAACT97450.027.16906419
Produced by FastQC (version 0.11.2)