FastQCFastQC Report
Wed 4 May 2016
G131-M1_ATCACG_BC8GTYANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M1_ATCACG_BC8GTYANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15712483
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC429960.2736423008381298TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA66800.039.1358531
TCGTATG68200.036.93844643
CGTATGC68650.036.34327344
ACGTCTG72250.036.1896115
ACACGTC72700.035.6874313
ATCGGAA73850.035.335352
ATCACGA70650.035.07951434
CGTCTGA74200.034.84930416
CTCGTAT69600.034.04911842
CACGTCT78650.033.1592414
TCACGAT67050.032.9289735
ACGATCT64000.032.7435437
CACACGT80700.032.61839312
CGGAAGA82450.031.5742764
TCGGAAG85250.031.1263643
CACGATC68100.030.80625536
CATCACG83100.030.4457333
TCTCGTA72200.029.23029741
CGATCTC73350.028.9257438
GCACACG91850.028.80415511
Produced by FastQC (version 0.11.2)