FastQCFastQC Report
Wed 4 May 2016
G131-M1_ATCACG_BC8GTYANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M1_ATCACG_BC8GTYANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15712483
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG595130.37876254185923386Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG77650.035.0059334
TCGTGTA77150.034.90486514
GTAGATC78250.034.5737431
CGTCGTG78950.034.41647312
TCTCGGT79750.034.0841536
GCGTCGT79850.034.0564411
ATCTCGG79500.034.02513535
CGTGTAG79650.033.9422115
GTCGTGT83250.032.66523413
AGAGCGT84400.032.4863248
GGTCGCC84300.032.2738743
GATCGGA84500.032.2658771
AGCGTCG84300.032.102710
GTCGCCG84950.031.87432544
GAGTGTA87500.031.19888927
GTGGTCG87250.031.05622541
GTAGGGA86750.030.98425918
ATCGGAA87400.030.9685882
TGGTCGC87800.030.9619842
GAGCGTC88950.030.5983099
Produced by FastQC (version 0.11.2)